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A
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K
Analytical Characterization
Primary Structure Analysis
- N-terminal Sequencing
- Amino acid composition
- Extinction coefficient determination
- Peptide mapping and sequencing
- Intact Mass
- Sulhydryl groups and disulfide mapping
- Conjugate Site analysis
Higher Order Structure Analysis
- Circular Dichroism (CD) spectroscopy
- Fourier Transform Infrared (FTIR) spectroscopy
- 1D and 2D NMR
- Differential Scanning Calorimetry (DSC)
- Fluorescence spectroscopy
- Free cysteine analysis ( Ellman’s Method)
Product and Size Related Variants
- Deamidation, oxidation, sequence variants and PTMs by MS
- Analytical Ultracentrifugation ( AUC)
- SEC-MALS ( Molecular weight determination)
- SDS-PAGE
- Dynamic Light Scattering (DLS)
Functional Characteristics
- Surface Plasmon resonance (SPR) based receptor binding assay
General Properties
- Visual Appearance
- Extractable Volume
- Protein Concentration
- pH
- Osmolality